Publications


2020

  • Buldum, G., Tsipa, A., & Mantalaris, A. (2020). “Linking engineered gene circuit kinetic modeling to cellulose biosynthesis prediction in escherichia coli: Toward bioprocessing of microbial cell factories”. Industrial and Engineering Chemistry Research, 59(10), 4659-4669. doi:10.1021/acs.iecr.9b05847
  • Doron, G., Klontzas, M. E., Mantalaris, A., Guldberg, R. E., & Temenoff, J. S. (2020). “Multiomics characterization of mesenchymal stromal cells cultured in monolayer and as aggregates”. Biotechnology and Bioengineering, doi:10.1002/bit.27317
  • Karakostas, P., Panoskaltsis, N., Mantalaris, A., & Georgiadis, M. C. (2020). “Optimization of CAR T-cell therapies supply chains.” Computers and Chemical Engineering, 139. https://doi.org/10.1016/j.compchemeng.2020.106913
  • Panoskaltsis, N., McCarthy, N. E., & Knight, S. C. (2020). “Myelopoiesis of acute inflammation: lessons from TGN1412-induced cytokine storm.” Cancer Immunology, Immunotherapy. https://doi.org/10.1007/s00262-020-02702-9
  • Tsipa, A., Pitt, J. A., Banga, J. R., & Mantalaris, A. (2020). “A dual-parameter identification approach for data-based predictive modeling of hybrid gene regulatory network-growth kinetics in Pseudomonas putida mt-2.” Bioprocess and Biosystems Engineering, 43(9), 1671–1688. https://doi.org/10.1007/s00449-020-02360-2
2019

  • Allenby, M. C., Panoskaltsis, N., Tahlawi, A., Dos Santos, S. B., & Mantalaris, A. (2019). “Dynamic human erythropoiesis in a three-dimensional perfusion bone marrow biomimicry”. Biomaterials, 188, 24-37. doi:10.1016/j.biomaterials.2018.08.020
  • Devito, L., Klontzas, M. E., Cvoro, A., Galleu, A., Simon, M., Hobbs, C., . . . Ilic, D. (2019). “Comparison of human isogeneic Wharton’s jelly MSCs and iPSC-derived MSCs reveals differentiation-dependent metabolic responses to IFNG stimulation”. Cell Death and Disease, 10(4) doi:10.1038/s41419-019-1498-0
  • Grilo, A. L., & Mantalaris, A. (2019). “A predictive mathematical model of cell cycle, metabolism, and apoptosis of monoclonal antibody-producing GS–NS0 cells”. Biotechnology Journal, 14(11) doi:10.1002/biot.201800573
  • Grilo, A. L., & Mantalaris, A. (2019). “Apoptosis: A mammalian cell bioprocessing perspective”. Biotechnology Advances, 37(3), 459-475. doi:10.1016/j.biotechadv.2019.02.012
  • Grilo, A. L., & Mantalaris, A. (2019). “The increasingly human and profitable monoclonal antibody market”. Trends in Biotechnology, 37(1), 9-16. doi:10.1016/j.tibtech.2018.05.014
  • György, R., Klontzas, M. E., Kostoglou, M., Panoskaltsis, N., Mantalaris, A., & Georgiadis, M. C. (2019). “Capturing mesenchymal stem cell heterogeneity during osteogenic differentiation: An experimental-modeling approach”. Industrial and Engineering Chemistry Research, 58(31), 13900-13909. doi:10.1021/acs.iecr.9b01988
  • Kappatou, C. D., Altunok, O., Mhamdi, A., Mantalaris, A., & Mitsos, A. (2019). “Sequential and simultaneous optimization strategies for increased production of monoclonal antibodies” Computer Aided Chemical Engineering, 46,1021-1026. doi:10.1016/B978-0-12-818634-3.50171-5
  • Klontzas, M. E., Reakasame, S., Silva, R., Morais, J. C. F., Vernardis, S., MacFarlane, R. J., . . . Mantalaris, A. (2019). “Oxidized alginate hydrogels with the GHK peptide enhance cord blood mesenchymal stem cell osteogenesis: A paradigm for metabolomics-based evaluation of biomaterial design”. Acta Biomaterialia, 88, 224-240. doi:10.1016/j.actbio.2019.02.017
  • Mortera-Blanco, T., Rende, M., Panoskaltsis, N., & Mantalaris, A. (2019). “Tissue engineering of normal and abnormal bone marrow”. Comprehensive biotechnology (pp. 225-235) doi:10.1016/B978-0-12-801238-3.99810-6
  • Tahlawi, A., Klontzas, M. E., Allenby, M. C., Morais, J. C. F., Panoskaltsis, N., & Mantalaris, A. (2019). “RGD-functionalized polyurethane scaffolds promote umbilical cord blood mesenchymal stem cell expansion and osteogenic differentiation”. Journal of Tissue Engineering and Regenerative Medicine, 13(2), 232-243. doi:10.1002/term.2784
2018

  • Allenby, M. C., Tahlawi, A., Morais, J. C. F., Li, K., Panoskaltsis, N., & Mantalaris, A. (2018). “Ceramic hollow fibre constructs for continuous perfusion and cell harvest from 3D hematopoietic organoids”. Stem Cells International, 2018 doi:10.1155/2018/6230214
  • Buldum, G., Bismarck, A., & Mantalaris, A. (2018). “Recombinant biosynthesis of bacterial cellulose in genetically modified escherichia coli”. Bioprocess and Biosystems Engineering, 41(2), 265-279. doi:10.1007/s00449-017-1864-1
  • Kappatou, C. D., Mhamdi, A., Campano, A. Q., Mantalaris, A., & Mitsos, A. (2018). “Model-based dynamic optimization of monoclonal antibodies production in semibatch operation – use of reformulation techniques”. Industrial and Engineering Chemistry Research, 57(30), 9915-9924. doi:10.1021/acs.iecr.7b05357
  • Misener, R., Allenby, M. C., Fuentes-Garí, M., Gupta, K., Wiggins, T., Panoskaltsis, N., . . . Mantalaris, A. (2018). “Stem cell biomanufacturing under uncertainty: A case study in optimizing red blood cell production”. AIChE Journal, 64(8), 3011-3022. doi:10.1002/aic.16042
  • Quiroga-Campano, A. L., Panoskaltsis, N., & Mantalaris, A. (2018). “Energy-based culture medium design for biomanufacturing optimization: A case study in monoclonal antibody production by GS-NS0 cells”. Metabolic Engineering, 47, 21-30. doi:10.1016/j.ymben.2018.02.013
  • Totti, S., Allenby, M. C., Dos Santos, S. B., Mantalaris, A., & Velliou, E. G. (2018). “A 3D bioinspired highly porous polymeric scaffolding system for: In vitro simulation of pancreatic ductal adenocarcinoma”. RSC Advances, 8(37), 20928-20940. doi:10.1039/c8ra02633e
  • Tsipa, A., Koutinas, M., Usaku, C., & Mantalaris, A. (2018). “Optimal bioprocess design through a gene regulatory network – growth kinetic hybrid model: Towards replacing monod kinetics”. Metabolic Engineering, 48, 129-137. doi:10.1016/j.ymben.2018.04.023
2017

  • Allenby, M. C., Misener, R., Panoskaltsis, N., & Mantalaris, A. (2017). “A quantitative three-dimensional image analysis tool for maximal acquisition of spatial heterogeneity data”. Tissue Engineering – Part C: Methods, 23(2), 108-117. doi:10.1089/ten.tec.2016.0413
  • Cha, J. M., Mantalaris, A., Jung, S., Ji, Y., Bang, O. Y., & Bae, H. (2017). “Mesoderm lineage 3D tissue constructs are produced at large-scale in a 3D stem cell bioprocess”. Biotechnology Journal, 12(9) doi:10.1002/biot.201600748
  • Győrgy, R., Klontzas, M. E., Kostoglou, M., Panoskaltsis, N., Georgiadis, M. C., & Mantalaris, A. (2017). “An integrated experimental-modelling approach of mesenchymal stem cell bioprocess towards osteogenic differentiation”. IFAC-PapersOnLine, 50(1), 9877-9882. doi:10.1016/j.ifacol.2017.08.1603
  • Győrgy, R., Klontzas, M. E., Kostoglou, M., Panoskaltsis, N., Mantalaris, A., & Georgiadis, M. C. (2017). “A population balance model for stem cell differentiation bioprocesses” Computer Aided Chemical Engineering, 40, 2761-2766. doi:10.1016/B978-0-444-63965-3.50462-1
  • Harrison, C. N., Mead, A. J., Panchal, A., Fox, S., Yap, C., Gbandi, E., . . . McMullin, M. F. (2017). “Ruxolitinib vs best available therapy for et intolerant or resistant to hydroxycarbamide”. Blood, 130(17), 1889-1897. doi:10.1182/blood-2017-05-785790
  • Kang, Y., Georgiou, A. I., MacFarlane, R. J., Klontzas, M. E., Heliotis, M., Tsiridis, E., & Mantalaris, A. (2017). “Fibronectin stimulates the osteogenic differentiation of murine embryonic stem cells”. Journal of Tissue Engineering and Regenerative Medicine, 11(7), 1929-1940. doi:10.1002/term.2090
  • Kappatou, C. D., Mhamdi, A., Campano, A. Q., Mantalaris, A., & Mitsos, A. (2017). “Dynamic optimization of the production of monoclonal antibodies in semi-batch operation”. Computer Aided Chemical Engineering, 40, 2161-2166. doi:10.1016/B978-0-444-63965-3.50362
  • Klontzas, M. E., Vernardis, S. I., Heliotis, M., Tsiridis, E., & Mantalaris, A. (2017). “Metabolomics analysis of the osteogenic differentiation of umbilical cord blood mesenchymal stem cells reveals differential sensitivity to osteogenic agents”. Stem Cells and Development, 26(10), 723-733. doi:10.1089/scd.2016.0315
  • Papathanasiou, M. M., Quiroga-Campano, A. L., Steinebach, F., Elviro, M., Mantalaris, A., & Pistikopoulos, E. N. (2017). “Advanced model-based control strategies for the intensification of upstream and downstream processing in mAb production”. Biotechnology Progress, 33(4), 966-988. doi:10.1002/btpr.2483
  • Papathanasiou, M. M., Steinebach, F., Morbidelli, M., Mantalaris, A., & Pistikopoulos, E. N. (2017). “Intelligent, model-based control towards the intensification of downstream processes”. Computers and Chemical Engineering, 105, 173-184. doi:10.1016/j.compchemeng.2017.01.005
  • Totti, S., Vernardis, S. I., Meira, L., Pérez-Mancera, P. A., Costello, E., Greenhalf, W., . . . Velliou, E. G. (2017). “Designing a bio-inspired biomimetic in vitro system for the optimization of ex vivo studies of pancreatic cancer”. Drug Discovery Today, 22(4), 690-701. doi:10.1016/j.drudis.2017.01.012
  • Tsipa, A., Koutinas, M., Vernardis, S., & Mantalaris, A. (2017). “The impact of succinate trace on pWW0 and ortho-cleavage pathway transcription in pseudomonas putida mt-2 during toluene biodegradation”. Bioresource Technology, 234, 397-405. doi:10.1016/j.biortech.2017.03.082
  • Vernardis, S. I., Terzoudis, K., Panoskaltsis, N., & Mantalaris, A. (2017). “Human embryonic and induced pluripotent stem cells maintain phenotype but alter their metabolism after exposure to ROCK inhibitor”. Scientific Reports, 7 doi:10.1038/srep42138
2016

  • Campano, A. Q., Papathanasiou, M. M., Pistikopoulos, E. N., & Mantalaris, A. (2016). “A predictive model for energy metabolism and ATP balance in mammalian cells: Towards the energy-based optimization of mAb production” Computer Aided Chemical Engineering, 38, 1581-1586. doi:10.1016/B978-0-444-63428-3.50268-X
  • Chaudhry, M. S., Gilmour, K. C., House, I. G., Layton, M., Panoskaltsis, N., Sohal, M., . . . Voskoboinik, I. (2016). “Missense mutations in the perforin (PRF1) gene as a cause of hereditary cancer predisposition.” OncoImmunology, 5(7) doi:10.1080/2162402X.2016.1179415
    Klontzas, M. E., Kenanidis, E. I., MacFarlane, R. J., Michail, T., Potoupnis, M. E., Heliotis, M., . . . Tsiridis, E. (2016). “Investigational drugs for fracture healing: Preclinical & clinical data”. Expert Opinion on Investigational Drugs, 25(5), 585-596. doi:10.1517/13543784.2016.1161757
  • Kostoglou, M., Fuentes-Garí, M., García-Münzer, D., Georgiadis, M. C., Panoskaltsis, N., Pistikopoulos, E. N., & Mantalaris, A. (2016). A comprehensive mathematical analysis of a novel multistage population balance model for cell proliferation”. Computers and Chemical Engineering, 91, 157-166. doi:10.1016/j.compchemeng.2016.02.012
  • Papathanasiou, M. M., Avraamidou, S., Oberdieck, R., Mantalaris, A., Steinebach, F., Morbidelli, M., . . . Pistikopoulos, E. N. (2016). “Advanced control strategies for the multicolumn countercurrent solvent gradient purification process”. AIChE Journal, 62(7), 2341-2357. doi:10.1002/aic.15203
  • Papathanasiou, M. M., Mantalaris, A., & Pistikopoulos, E. N. (2016). “Advanced control strategies for a periodic, two-column chromatographic process”. Paper presented at the 2016 20th IEEE International Conference on Automation, Quality and Testing, Robotics, AQTR 2016 – Proceedings, doi:10.1109/AQTR.2016.7501359
  • Papathanasiou, M. M., Oberdieck, R., Avraamidou, S., Nascu, I., Mantalaris, A., & Pistikopoulos, E. N. (2016). “Development of advanced control strategies for periodic systems: An application to chromatographic separation processes”. Paper presented at the Proceedings of the American Control Conference, , 2016-July 4175-4180. doi:10.1109/ACC.2016.7525578
  • Papathanasiou, M. M., Oberdieck, R., Mantalaris, A., & Pistikopoulos, E. N. (2016). “Computational tools for the advanced control of periodic processes – application to a chromatographic separation” Computer Aided Chemical Engineering, 38, 1665-1670. doi:10.1016/B978-0-444-63428-3.50282-4
  • Papathanasiou, M. M., Quiroga-Campano, A. L., Oberdieck, R., Mantalaris, A., & Pistikopoulos, E. N. (2016). “Development of advanced computational tools for the intensification of monoclonal antibody production” Computer Aided Chemical Engineering, 38, 1659-1664. doi:10.1016/B978-0-444-63428-3.50281-2
  • Papathanasiou, M. M., Sun, M., Oberdieck, R., Mantalaris, A., & Pistikopoulos, E. N. (2016). “A centralized/decentralized control approach for periodic systems with application to chromatographic separation processes”. IFAC-PapersOnLine, 49(7), 159-164. doi:10.1016/j.ifacol.2016.07.233
  • Reissis, D., Tang, Q. O., Cooper, N. C., Carasco, C. F., Gamie, Z., Mantalaris, A., & Tsiridis, E. (2016). “Current clinical evidence for the use of mesenchymal stem cells in articular cartilage repair”. Expert Opinion on Biological Therapy, 16(4), 535-557. doi:10.1517/14712598.2016.1145651
  • Savvopoulos, S., Misener, R., Panoskaltsis, N., Pistikopoulos, E. N., & Mantalaris, A. (2016). “A personalized framework for dynamic modeling of disease trajectories in chronic lymphocytic leukemia”. IEEE Transactions on Biomedical Engineering, 63(11), 2396-2404. doi:10.1109/TBME.2016.2533658
  • Severn, C. E., Macedo, H., Eagle, M. J., Rooney, P., Mantalaris, A., & Toye, A. M. (2016). “Polyurethane scaffolds seeded with CD34 + cells maintain early stem cells whilst also facilitating prolonged egress of haematopoietic progenitors”. Scientific Reports, 6 doi:10.1038/srep32149
  • Tsipa, A., Koutinas, M., Pistikopoulos, E. N., & Mantalaris, A. (2016). “Transcriptional kinetics of the cross-talk between the ortho-cleavage and TOL pathways of toluene biodegradation in pseudomonas putida mt-2″. Journal of Biotechnology, 228, 112-123. doi:10.1016/j.jbiotec.2016.03.053
  • Zubairi, S. I., Mantalaris, A., Bismarck, A., & Aizad, S. (2016). “Polyhydroxyalkanoates (PHAs) for tissue engineering applications: Biotransformation of palm oil mill effluent (POME) to value-added polymers”. Jurnal Teknologi, 78(1), 13-29. doi:10.11113/jt.v78.4042
2015

  • Burnett, A. K., Russell, N., Hills, R. K., Panoskaltsis, N., Khwaja, A., Hemmaway, C., . . . Milligan, D. (2015). “A randomised comparison of the novel nucleoside analogue sapacitabine with low-dose cytarabine in older patients with acute myeloid leukaemia”. Leukemia, 29(6), 1312-1319. doi:10.1038/leu.2015.38
  • Dennis, M., Russell, N., Hills, R. K., Hemmaway, C., Panoskaltsis, N., McMullin, M. -., . . . Burnett, A. K. (2015). “Vosaroxin and vosaroxin plus low-dose ara-C (LDAC) vs low-dose ara-C alone in older patients with acute myeloid leukemia”. Blood, 125(19), 2923-2932. doi:10.1182/blood-2014-10-608117
  • Fauzi, I., Panoskaltsis, N., & Mantalaris, A. (2015). “In vitro differentiation of embryonic stem cells into hematopoietic lineage: Towards erythroid progenitor’s production” In: Turksen K. (eds) Embryonic Stem Cell Protocols. Methods in Molecular Biology, vol 1341. Humana Press, New York, NY. doi:10.1007/7651_2015_218
  • Fuentes-Gaŕ, M., Misener, R., Georgiadis, M. C., Kostoglou, M., Panoskaltsis, N., Pistikopoulos, E. N., & Mantalaris, A. (2015). “Chemotherapy optimization in leukemia: Selecting the right mathematical models for the right biological processes”. IFAC-PapersOnLine, 28(20), 534-539. doi:10.1016/j.ifacol.2015.10.196
  • Fuentes-Garí, M., Misener, R., García-Munzer, D., Velliou, E., Georgiadis, M. C., Kostoglou, M., . . . Mantalaris, A. (2015). “A mathematical model of subpopulation kinetics for the deconvolution of leukaemia heterogeneity”. Journal of the Royal Society Interface, 12(108) doi:10.1098/rsif.2015.0276
  • Fuentes-Garí, M., Misener, R., Georgiadis, M. C., Kostoglou, M., Panoskaltsis, N., Mantalaris, A., & Pistikopoulos, E. N. (2015). “Selecting a differential equation cell cycle model for simulating leukemia treatment”. Industrial and Engineering Chemistry Research, 54(36), 8847-8859. doi:10.1021/acs.iecr.5b01150
  • Fuentes-Garí, M., Misener, R., Pefani, E., García-Münzer, D., Kostoglou, M., Georgiadis, M. C., . . . Mantalaris, A. (2015). “Cell cycle model selection for leukemia and its impact in chemotherapy outcomes”. Computer Aided Chemical Engineering, 37, 2159-2164, doi:10.1016/B978-0-444-63576-1.50054-6
  • Fuentes-Garí, M., Velliou, E., Misener, R., Pefani, E., Rende, M., Panoskaltsis, N., . . . Pistikopoulos, E. N. (2015). “A systematic framework for the design, simulation and optimization of personalized healthcare: Making and healing blood”. Computers and Chemical Engineering, 81, 80-93. doi:10.1016/j.compchemeng.2015.03.008
  • García Münzer, D. G., Ivarsson, M., Usaku, C., Habicher, T., Soos, M., Morbidelli, M., . . . Mantalaris, A. (2015). “An unstructured model of metabolic and temperature dependent cell cycle arrest in hybridoma batch and fed-batch cultures”. Biochemical Engineering Journal, 93, 260-273. doi:10.1016/j.bej.2014.10.013
  • García Münzer, D. G., Kostoglou, M., Georgiadis, M. C., Pistikopoulos, E. N., & Mantalaris, A. (2015). “Cyclin and DNA distributed cell cycle model for GS-NS0 cells”. PLoS Computational Biology, 11(2) doi:10.1371/journal.pcbi.1004062
  • Kiparissides, A., Pistikopoulos, E. N., & Mantalaris, A. (2015). “On the model-based optimization of secreting mammalian cell (GS-NS0) cultures”. Biotechnology and Bioengineering, 112(3), 536-548. doi:10.1002/bit.25457
  • Klontzas, M. E., Kenanidis, E. I., Heliotis, M., Tsiridis, E., & Mantalaris, A. (2015). “Bone and cartilage regeneration with the use of umbilical cord mesenchymal stem cells”. Expert Opinion on Biological Therapy, 15(11), 1541-1552. doi:10.1517/14712598.2015.1068755
  • Kostoglou, M., Fuentes-Garí, M., García-Münzer, D., Georgiadis, M. C., Panoskaltsis, N., Pistikopoulos, E. N., & Mantalaris, A. (2015). Mathematical analysis of multistage population balances for cell growth and death. Computer Aided Chemical Engineering, 37, 2105-2110. doi:10.1016/B978-0-444-63576-1.50045-5
  • Papathanasiou, M. M., Campano, A. Q., Steinebach, F., Mueller-Spaeth, T., Morbidelli, M., Mantalaris, A., & Pistikopoulos, E. N. (2015). “424226 Advanced control strategies towards the intensification of monoclonal antibody production”. Paper presented at the Process Development Division 2015 – Core Programming Area at the 2015 AIChE Annual Meeting, 429-431.
  • Papathanasiou, M. M., Campano, A. Q., Steinebach, F., Mueller-Spaeth, T., Morbidelli, M., Mantalaris, A., & Pistikopoulos, E. N. (2015). “Advanced control strategies towards the intensification of monoclonal antibody production”. Paper presented at the Computing and Systems Technology Division 2015 – Core Programming Area at the 2015 AIChE Annual Meeting, , 2 714-716.
  • Papathanasiou, M. M., Steinebach, F., Stroehlein, G., Müller-Späth, T., Nascu, I., Oberdieck, R., . . . Pistikopoulos, E. N. (2015). “A control strategy for periodic systems – application to the twin-column MCSGP”. Computer Aided Chemical Engineering, 37, 1505-1510. doi:10.1016/B978-0-444-63577-8.50096-6
  • Papathanasiou, M. M., Sun, M., Steinebach, F., Müller-Späth, T., Morbidelli, M., Mantalaris, A., & Pistikopoulos, E. N. (2015). “A centralized/decentralized control approach for the multicolumn countercurrent solvent gradient purification (MCSGP) process”. Paper presented at the Computing and Systems Technology Division 2015 – Core Programming Area at the 2015 AIChE Annual Meeting, , 1 355-357.
  • Savvopoulos, S., Misener, R., Panoskaltsis, N., Pistikopoulos, E. N., & Mantalaris, A. (2015). “Global sensitivity analysis for a model of B-cell chronic lymphocytic leukemia disease trajectories.” Computer Aided Chemical Engineering, 37, 185-190. doi:10.1016/B978-0-444-63578-5.50026-8
  • Tsipa, A., Koutinas, M., Pistikopoulos, E. N., & Mantalaris, A. (2015). “Prediction of double substrate microbial growth kinetics through transcriptional regulation: An integrated experimental/modelling approach on pseudomonas putida mt-2″. Paper presented at the Food, Pharmaceutical and Bioengineering Division 2015 – Core Programming Area at the 2015 AIChE Meeting, , 2 763-768.
  • Velliou, E. G., Dos Santos, S. B., Papathanasiou, M. M., Fuentes-Gari, M., Misener, R., Panoskaltsis, N., . . . Mantalaris, A. (2015). “Towards unravelling the kinetics of an acute myeloid leukaemia model system under oxidative and starvation stress: A comparison between two- and three-dimensional cultures”. Bioprocess and Biosystems Engineering, 38(8), 1589-1600. doi:10.1007/s00449-015-1401-z
  • Zavitsanou, S., Mantalaris, A., Georgiadis, M. C., & Pistikopoulos, E. N. (2015). “In silico closed-loop control validation studies for optimal insulin delivery in type 1 diabetes”. IEEE Transactions on Biomedical Engineering, 62(10), 2369-2378. doi:10.1109/TBME.2015.2427991
  • Zubairi, S. I., Bismarck, A., & Mantalaris, A. (2015). “The effect of surface heterogeneity on wettability of porous three dimensional (3-D) scaffolds of poly(3-hydroxybutyric acid) (PHB) and poly(3-hydroxybutyric-co-3-hydroxyvaleric acid) (PHBV)”. Jurnal Teknologi, 75(1), 305-312. doi:10.11113/jt.v75.3960
  • Zubairi, S. I., Mantalaris, A., & Bismarck, A. (2015). “Pore interconnectivity analysis of porous three dimensional scaffolds of poly(3-hydroxybutyric acid) (PHB) and poly(3-hydroxybutyric-co-3-hydroxyvaleric acid) (PHBV) through non-invasive color staining method”. Sains Malaysiana, 44(9), 1351-1356. doi:10.17576/jsm-2015-4409-18